Leaf diffusional capacity largely contributes to the reduced photosynthesis in rice plants under magnesium deficiency.

Numerous studies have clarified the impacts of magnesium (Mg) on leaf photosynthesis from the perspectives of protein synthesis, enzymes activation and carbohydrate partitioning. However, it still remains largely unknown how stomatal and mesophyll conductances (gs and gm, respectively) are regulated by Mg. In the present study, leaf gas exchanges, leaf hydraulic parameters, leaf structural traits and cell wall composition were examined in rice plants grown under high and low Mg treatments to elucidate the impacts of Mg on gs and gm. Our results showed that reduction of leaf photosynthesis under Mg deficiency was mainly caused by the decreased gm, followed by reduced leaf biochemical capacity and gs, and leaf outside-xylem hydraulic conductance (Kox) was the major factor restricting gs under Mg deficiency. Moreover, increased leaf hemicellulose, lignin and pectin contents and decreased cell wall effective porosity were observed in low Mg plants relative to high Mg plants. These results suggest that Kox and cell wall composition play important roles in regulating gs and gm, respectively, in rice plants under Mg shortages.

The sensitivity of photosynthesis to magnesium deficiency differs between rice (Oryza sativa L.) and cucumber (Cucumis sativus L.).

Magnesium is an essential macronutrient for plant photosynthesis, and in response to Mg deficiency, dicots appear more sensitive than monocots. Under Mg deficiency, we investigated the causes of differing photosynthetic sensitivities in a dicot and a monocot species. Rice (Oryza sativa L.) and cucumber (Cucumis sativus L.) were grown in hydroponic culture to explore their physiological responses to Mg deficiency stress. Both Mg-deficient rice and cucumber plants exhibited lower biomass, leaf area, Mg concentration, and chlorophyll content (Chl) compared with Mg-sufficient plants. However, a more marked decline in Chl and carotenoid content (Car) occurred in cucumber. A lower CO2 concentration in chloroplasts (C c) was accompanied by a decrease in the maximum rate of electron transport (J max) and the maximum rate of ribulose 1,5-bisphosphate carboxylation (V cmax), restricting CO2 utilization in Mg-deficient plants. Rice and cucumber photorespiration rate (P r) increased under Mg deficiency. Additionally, for cucumber, Car and non-photochemical quenching (NPQ) were reduced under lower Mg supply. Meanwhile, cucumber Mg deficiency significantly increased the fraction of absorbed light energy dissipated by an additional quenching mechanism (Φf,D). Under Mg deficiency, suppressed photosynthesis was attributed to comprehensive restrictions of mesophyll conductance (g m), J max, and V cmax. Cucumber was more sensitive to Mg deficiency than rice due to lower NPQ, higher rates of electron transport to alternative pathways, and subsequently, photooxidation damage.

Magnesium deficiency stress in rice can be alleviated by partial nitrate nutrition supply.

The problem of nitrogen (N) excess and magnesium (Mg) deficiency in farmland is becoming more common, severe, and widespread in southern China. Magnesium is known to be an essential nutrient for higher plants; however, the physiological responses of field crops to Mg deficiency, particularly to its interaction with N forms, remain largely unknown. In this study, a hydroponic experiment was conducted using three Mg levels (0.01, 1.00, and 5.00 mM) and three nitrate/ammonium ratios (NO3-/NH4+ of 0/100, 25/75, and 50/50) under greenhouse conditions. The results show that Mg deficiency (0.01 mM) could result in yellow leaves, dwarf plants, and fewer tillers during rice growth. Furthermore, Mg deficiency induced a major reduction in root morphology and activity, photosynthetic properties, and nutrient accumulation, while it resulted in a clear increase in malondialdehyde, superoxide dismutase, peroxidase, and catalase activities in rice. However, under Mg-deficiency stress, the supply of partial NO3- led to a significant drop in these antioxidant enzyme activities. Moreover, partial NO3- supply significantly improved the net photosynthetic rate, transpiration rate, stomatal conductance, and intercellular CO2 concentrations under Mg-deficiency conditions. In particular, the supply of partial NO3- dramatically promoted the growth of the root system, boosted the occurrence of lateral roots, and enhanced root vitality under Mg-deficiency stress. Additionally, the supply of partial NO3- led to significant increases in dry weight and N and Mg contents under Mg deficiency. The results of this study suggest that the symptoms of Mg-deficiency stress in rice can be alleviated by partial NO3- supply.

Anatomically induced changes in rice leaf mesophyll conductance explain the variation in photosynthetic nitrogen use efficiency under contrasting nitrogen supply.

BACKGROUND: The ratio of CO2 mesophyll conductance (gm) to Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) content has been suggested to positively affect photosynthetic nitrogen use efficiency (PNUE). The anatomical basis of gm has been quantified, but information on the relationship between cell-level anatomies and PNUE is less advanced. Here, hydroponic experiments were conducted in rice plants supplied with ammonium (NH4+) and nitrate (NO3-) under three N levels (low, 0.71 mM; intermediate, 2.86 mM; high, 7.14 mM) to investigate the gas exchange parameters, leaf anatomical structure and PNUE. RESULTS: The results showed a lower PNUE in plants supplied with high nitrogen and NH4+, which was positively correlated with the gm/Rubisco ratio. A one-dimensional within-leaf model revealed that the resistance to CO2 diffusion in the liquid phase (rliq) dominated the overall mesophyll resistance (rm), in which CO2 transfer resistance in the cell wall, cytoplasm and stroma were significantly affected by nitrogen supply. The chloroplast surface area exposed to intercellular space (Sc) per Rubisco rather than the gm/Sc ratio was positively correlated with PNUE and was thus considered a key component influencing PNUE. CONCLUSION: In conclusion, our study emphasized that Sc was the most important anatomical trait in coordinating gm and PNUE with contrasting N supply.

Leaf photosynthesis is mediated by the coordination of nitrogen and potassium: The importance of anatomical-determined mesophyll conductance to CO2 and carboxylation capacity.

Both nitrogen (N) and potassium (K) have been widely studied in maintaining efficient photosynthesis and plant growth. However, the mechanisms underlying the photosynthetic response to their interaction remain unclear. This study assessed the effects of N and K supply on photosynthetic limitations and the corresponding changes in anatomical structures in leaves of rice (Oryza sativa L.) plants, grown hydroponically under different levels of N and K in a greenhouse. Results revealed that a suitable leaf N/K ratio (2.99-3.10) maintain a high rate of photosynthesis (A). The A under N and/or K deficiency was primarily limited by mesophyll conductance (gm) and RuBP carboxylation in biochemical processes. The decline of gm in N- or K-starved leaves was mostly resulted from low surface area of chloroplasts exposed to intercellular airspaces (Sc) and high mesophyll cell wall thickness. Synergistic effects of N and K on gm were reflected in leaf anatomical structure, especially their coordinated roles in enhancing Sc. The enhanced photosynthesis in plants with coordinated supply of N and K was caused by the balance of RuBP carboxylation and regeneration. These results highlight the synergistic effect of N and K on leaf photosynthesis, which are mainly reflected in facilitating anatomical-determined gm and carboxylation capacity.

Potassium mediates coordination of leaf photosynthesis and hydraulic conductance by modifications of leaf anatomy.

Typical symptoms of potassium deficiency, characterized as chlorosis or withered necrosis, occur concomitantly with downregulated photosynthesis and impaired leaf water transport. However, the prominent limitations and mechanisms underlying the concerted decreases of leaf photosynthesis and hydraulic conductance are poorly understood. Monocots and dicots were investigated based on responses of photosynthesis and hydraulic conductance and their components and the correlated anatomical determinants to potassium deficiency. We found a conserved pattern in which leaf photosynthesis and hydraulic conductance concurrently decreased under potassium starvation. However, monocots and dicots showed two different hydraulic-redesign strategies: Dicots tended to show a decreased minor vein density, whereas monocots reduced the size of the bundle sheath and its extensions, rather than the minor vein density; both of these strategies may restrain xylem and outside-xylem hydraulic conductance. Additionally, potassium-deprived leaves developed with fewer mesophyll cell-to-cell connections, leading to a reduced area being available for liquid-phase flow. Further quantitative analysis revealed that mesophyll conductance to CO2 and outside-xylem hydraulic resistance were the major contributors to photosynthetic limitation and increased hydraulic resistance, at more than 50% and 60%, respectively. These results emphasize the importance of potassium in the coordinated regulation of leaf photosynthesis and hydraulic conductance through modifications of leaf anatomy.

Improving rice population productivity by reducing nitrogen rate and increasing plant density.

In terms of tillering potential, the aboveground portions of rice are significantly influenced by the nitrogen level (NL) and transplant density (TD). To obtain a suitable combination of NL and TD, five NLs (0, 90, 180, 270 and 360 kg ha-1) and two TDs [high density (HD), 32.5×104 hills ha-1; low density (LD), 25.5×104 hills ha-1] were used in the rice experiments during 2012 to 2014, in Jiangsu, China. The results showed the highest grain yield of rice obtained at HD and LD when N supply was 180 and 270 kg ha-1, respectively. That's because there are more tillers per unit area, a larger leaf biomass fraction of total aboveground biomass, a larger leaf area index (LAI) and a larger canopy photosynthesis potential (CPP) at HD. It can be concluded that, higher rice planting densities resulted in less N inputs, while more N is needed to improve single plant actual tiller ability under low density to offset the reduced planting density. When the NL was more than 180 kg ha-1, the actual tillering ability of a single plant at LD was 20% more than that at HD. Based on these results, the supply of 1 kg N can be replaced by adding approximately 1000 planting hills per hectare. Therefore, adjusting the transplant density could be an efficient method to reduce the amount of nitrogen fertilizer and increase the nitrogen fertilizer use efficiency, which is very conducive to the sustainable development of agriculture.

The rice production practices of high yield and high nitrogen use efficiency in Jiangsu, China.

To face the great challenges of ensuring food security and environmental sustainability, agricultural production must be improved by high yield and high resource utilization efficiency (HYHE). We recently addressed this challenge and evaluated yield potential by surveying 735 farmers in 2008-2012 and then conducting 6 rice field experiments in 2008-2013 with large demonstration areas in 2010-2013 aimed to actualize the HYHE in Jiangsu Province, China. The survey result showed that the averaged N rate, grain yield and N partial factor productivity (PFPN) of the farmers were 336.7 kg ha-1, 8131.8 kg ha-1 and 24.2 kg kg-1, respectively. Through controlling total N rates and adjusting the application timing, the yield and the PFPN of optimal N managements (OPT) were increased by 5.9% and 37.6% with 31.4% reduction in N supply amounts for 6 experimental sites, and the yield increased by 5.6% for large demonstration areas compared with farmers' fertilizer practices (FFP), respectively. In conclusion, although the soil properties of the different regions varied, HYHE could be achieved by regulating the N management practices, thus contributing to higher rice production and lower environmental costs from intensive agriculture in Jiangsu, China.